Regulatory

Part:BBa_K3753003:Design

Designed by: Jiaying Hu   Group: iGEM21_NJTech_China   (2021-10-13)


pTEF2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TEF2 maps on chromosome II in the yeast Saccharomyces cerevisiae. pTEF(TEF2) was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 1000bp sequence upstream of the TEF2 coding sequence, which is a putative TEF2 promoter.


Source

Saccharomyces cerevisiae

References

Dominik Mumberg and Rolf Müller and Martin Funk. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds[J]. Gene, 1995, 156(1) : 119-122.

Schirmaier F and Philippsen P. Identification of two genes coding for the translation elongation factor EF-1 alpha of S. cerevisiae.[J]. The EMBO journal, 1984, 3(13) : 3311-5.